Journal: Journal of neurochemistry
Article Title: Fabp1 GENE ABLATION INHIBITS HIGH FAT DIET-INDUCED INCREASE IN BRAIN ENDOCANNABINOIDS
doi: 10.1111/jnc.13890
Figure Lengend Snippet: Fabp1 gene ablation (LKO) differentially impacts the ability of high fat diet (HFD) to alter serum N-acylethanolamide (NAE) and 2-monoacylglycerol (2-MG) levels. All conditions were as in Figures 1 and and22 except that NAE and 2-MG were determined in serum by LC/MS as described in Materials and Methods. Panels refer to: (A) arachidonoylethanolamide (AEA), (B) oleoylethanolamide (OEA), (C) palmitoylethanolamide (PEA), (D) docosahexaenoylethanolamide (DHEA), (E) 2-arachidonoylglycerol (2-AG), (F) 2-oleoylglycerol (2-OG), and (G) 2-palmitoylglycerol (2-PG). Results are expressed as the molar ratio of each NAE or 2-MG in High-Fat/Control diet and presented as mean ± SEM (n = 8); *, P < 0.05 for LKO vs WT; #, P < 0.05 for female (F) vs male (M).
Article Snippet: Anti-rabbit fatty acid transport protein 1 (FATP-1; sc-25541), monoclonal anti-mouse fatty acid binding protein-3 (FABP3; sc-58275), as well as rabbit polyclonal anti-rabbit fatty acid binding protein-7 (FABP7; sc-30088), monoclonal anti-mouse N-acylethanolamide hydrolyzing acid amidase (NAAA; sc-100470), monoclonal anti-mouse β-Actin (sc-47778), rabbit polyclonal anti-monoacylglycerol lipase (MGL, sc-134789) and anti- diacylglycerol lipase α (DAGLα; sc-133307) were from Santa Cruz Biotech (Santa Cruz, CA).
Techniques: Liquid Chromatography with Mass Spectroscopy